Immobilization of ZnO-TiO2 Nanocomposite into Polyimidazolium Amphiphilic Chitosan Film, Targeting Improving Its Antimicrobial and Antibiofilm Applications

This study presents a green protocol for the fabrication of multifunctional smart nanobiocomposite (NBC) (ZnO-PIACSB-TiO2) secure antimicrobial and antibiofilm applications. First, shrimp shells were upgraded to polyimidazolium amphiphilic chitosan Schiff base (PIACSB) through series physicochemical processes. After that, PIACSB was used as an encapsulating coating agent manufacture hybrid NBC in situ by co-encapsulating ZnONPs TiO2NPs. The visual characteristics new investigated spectral, microscopic, electrical, thermal methods. indices revealed that newly synthesized, PIACSB-coated TiO2–ZnO nanocomposite is exciting antibiotic due its amazing activity (MIC/MBC→0.34/0.68 μg/mL, 0.20/0.40 0.15/0.30 μg/mL working against S. aureus, E. coli, P. aeruginosa, respectively) antifungal capabilities. Additionally, ZnO-PIACSB-TiO2 potential fighter bacterial biofilms, with results being superior those positive control (Cipro), which worked aureus (only 8.7% ± 1.9 biofilm growth), coli 1.4% 1.1 aeruginosa 0.85% 1.3 growth). Meanwhile, exhibits excellent biocompatibility, evidenced IC50 values both L929 HSF (135 143 µg/mL), are significantly higher than MIC doses (0.24–24.85 µg/mL) work all tested microbes, well uncoated (IC50 = 19.36 2.04 23.48 1.56 µg/mL). These findings imply film may offer promising food packaging additives address customer demand safe, eco-friendly products outstanding